Publications

6. Shutt, J. D.,Trivedi, U. H., Nicholls, J. A. (2021). Faecal metabarcoding reveals pervasive long-distance impacts of garden bird feeding. Proc. Roy. Soc. B.

Supplementary feeding of wildlife is widespread, being undertaken by more than half of households in many countries. However, the impact that these supplemental resources have is unclear, with impacts largely considered to be restricted to urban ecosystems. We reveal the pervasiveness of supplementary foodstuffs in the diet of a wild bird using metabarcoding of blue tit (Cyanistes caeruleus) faeces collected in early spring from a 220 km transect in Scotland with a large urbanization gradient. Supplementary foodstuffs were present in the majority of samples, with peanut (Arachis hypogaea) the single commonest (either natural or supplementary) dietary item. Consumption rates exhibited a distance decay from human habitation but remained high at several hundred metres from the nearest household and continued to our study limit of 1.4 km distant. Supplementary food consumption was associated with a near quadrupling of blue tit breeding density and a 5-day advancement of breeding phenology. We show that woodland bird species using supplementary food have increasing UK population trends, while species that do not, and/or are outcompeted by blue tits, are likely to be declining. We suggest that the impacts of supplementary feeding are larger and more spatially extensive than currently appreciated and could be disrupting population and ecosystem dynamics.

5. Der Weduwen, D., Keogan, K., Samplonius, J.M., Phillimore, A.B. and Shutt, J.D., 2021. The correlates of intraspecific variation in nest height and nest building duration in the Eurasian blue tit Cyanistes caeruleus. Journal of Avian Biology, 52(3).

Birds build nests primarily as a receptacle to lay their eggs in, but they can also provide secondary benefits including structural support, camouflage and adjustment of the microclimate surrounding the eggs and offspring. The factors underlying intraspecific variation in nest characteristics are poorly understood. In this study, we aim to identify the environmental factors that predict nest height variation and the duration of nest building in blue tits Cyanistes caeruleus, evaluating latitude, elevation, temperature and the timing of egg-laying as predictors of nest height, while also taking into account female and male parental identity. Using 713 nest height observations collected over a period of five years along a 220 km transect in Scotland, we found that if the annual mean timing of egg-laying was earlier, nests were taller. However, there was no correlation between nest height and elevation, latitude, the minimum temperature in the 14 days pre-egg-laying or the phenology of birds within a year. Female parental identity accounted for a large amount of variation in nest height, suggesting that individual behaviour has an influence on nest structure. We also found that nest building duration was shorter when egg laying occurred earlier in the year, and that across all observations taller nests took longer to build. Overall, our results show that blue tits are able to alter their nest characteristics based on environmental gradients like latitude (in the case of building duration) and the annual mean phenological variation of egg laying, and that birds build relatively taller nests faster.

4. Shutt, J. D., Nicholls, J. A.,Trivedi, U. H., Burgess, M. D., Stone, G. N., Hadfield, J. D. & Phillimore, A. B. (2020). Gradients in richness and turnover of a forest passerine’s diet prior to breeding: a mixed model approach applied to faecal metabarcoding data. Mol. Ecol.

Little is known about the dietary richness and variation of generalist insectivorous species, including birds, due primarily to difficulties in prey identification. Using faecal metabarcoding we provide the most comprehensive analysis of a passerine’s diet to date, identifying the relative magnitudes of biogeographic, habitat and temporal trends in the richness and turnover in diet of Cyanistes caeruleus (blue tit) along a 39‐site, 2° latitudinal transect in Scotland. Faecal samples were collected in 2014‐15 from adult birds roosting in nestboxes prior to nest building. DNA was extracted from 793 samples and we amplified COI and 16S minibarcodes. We identified 432 molecular operational taxonomic units (MOTUs) that correspond to putative dietary items. Most dietary items were rare, with Lepidoptera being the most abundant and taxon‐rich prey order. We present a statistical approach for estimation of gradients and inter‐sample variation in taxonomic richness and turnover using a generalised linear mixed model. We discuss the merits of this approach over existing tools and present methods for model‐based estimation of repeatability, taxon richness and Jaccard indices. We find that dietary richness increases significantly as spring advances, but changes little with elevation, latitude or local tree composition. In comparison, dietary composition exhibits significant turnover along temporal and spatial gradients and among sites. Our study shows the promise of faecal metabarcoding for inferring the macroecology of food webs, but we also highlight the challenge posed by contamination and make recommendations of laboratory and statistical practices to minimise its impact on inference.

3. Shutt, J. D., Cabello, I. B., Keogan. K., Leech, D. I., Samplonius, J. M., Whittle, L. Burgess, M. D., & Phillimore, A. B. (2019). The environmental predictors of spatiotemporal variation in the breeding phenology of a passerine bird. Proc. Roy. Soc. B.. (Media: BBC, The Times)

Establishing the cues or constraints that influence avian timing of breeding is key to accurate prediction of future phenology. This study aims to identify the aspects of the environment that predict the timing of two measures of breeding phenology (nest initiation and egg laying date) in an insectivorous woodland passerine, the blue tit (Cyanistes caeruleus). We analyse data collected from a 220km, 40-site transect over three years and consider spring temperatures, tree leafing phenology, invertebrate availability and photoperiod as predictors of breeding phenology. We find that mean night-time temperature in early spring is the strongest predictor of both nest initiation and lay date and suggest this finding is most consistent with temperature acting as a constraint on breeding activity. Birch budburst phenology significantly predicts lay date additionally to temperature, either as a direct cue or indirectly via a correlated variable. We use cross-validation to show that our model accurately predicts lay date in two further years, and find that similar variables predict lay date well across the UK national nest record scheme. This work refines our understanding of the principal factors influencing the timing of tit reproductive phenology, and suggests that temperature may have both a direct and indirect effect.

2. Shutt, J. D., Burgess, M. D., & Phillimore, A. B. (2019). A spatial perspective on the phenological distribution of the spring woodland caterpillar peak. American Naturalist.

A classic system for studying trophic mismatch focuses on the timing of the spring caterpillar peak in relation to the breeding time and productivity of woodland passerine birds. Most work has been conducted in single-site oak woodlands and little is known about how insights generalise to other woodland types or across space. Here we present the results of a three-year study on the species composition and temporal distribution of the spring caterpillar peak on different tree taxa across 40 woodland sites spanning two degrees of latitude in Scotland. We used molecular barcoding to identify 62 caterpillar species, with winter moth (Operophtera brumata) the most abundant, comprising a third of the sample. Oak (Quercus sp.) and willow (Salix sp.) hosted significantly higher caterpillar abundances than other tree taxa, with winter moth exhibiting similar trends and invariantly proportionate across tree taxa. Caterpillar peak phenology was broadly similar between tree taxa. While latitude had little effect, increasing elevation increased the height of the caterpillar peak and retarded timing by 3.7 days/100m. These findings extend our understanding of how mismatch may play out spatially, with caterpillar peak date varying with elevation, and tree taxa varying in the caterpillar resource that they host.

1. Shutt, J. D., Bolton, M., Cabello, I. B., Burgess, M. D., & Phillimore, A. B. (2018). The effects of woodland habitat and biogeography on blue tit (Cyanistes caeruleus) territory occupancy and productivity along a 220km transect. Ecography.

The nesting phenology and productivity of hole‐nesting woodland passerines, such as tit species (Paridae), has been the subject of many studies and played a central role in advancing our understanding of the causes and consequences of trophic mismatch. However, as most studies have been conducted in mature, oak‐rich (Quercus sp.) woodlands, it is unknown whether insights from such studies generalise to other habitats used by woodland generalist species. Here we applied spatial mixed models to data collected over three years (2014–2016) from 238 nestboxes across 40 sites – that vary in woodland habitat and elevation – along a 220 km transect in Scotland. We evaluate the importance of habitat, biogeography and food availability as predictors of mesoscale among‐site variation in blue tit Cyanistes caeruleus nestbox occupancy and two components of productivity (clutch size and fledging success). We found that habitat was not a significant predictor of occupancy or clutch size but that occupancy exhibited pronounced biogeographic trends, declining with increasing latitude and elevation. However, fledging success, defined as the proportion of a clutch that fledged, was positively correlated with site level availability of birch, oak and sycamore, and tree diversity. The lack of correspondence between the effects of habitat on fledging success versus occupancy and clutch size may indicate that blue tits do not accurately predict the future quality of their breeding sites when selecting territories and laying clutches. We found little evidence of spatial autocorrelation in occupancy or clutch size, whereas spatial autocorrelation in fledging success extends over multiple sites, albeit non‐significantly. Taken together, our findings suggest that the relationship between breeding decisions and breeding outcomes varies among habitats, and we urge caution when extrapolating inferences from one habitat to others.